Review



cd4 t lymphoid cell line supt  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    ATCC cd4 t lymphoid cell line supt
    Defect in proteolytic processing of HIV-1 Gag precursors in Myr− <t>CD4+</t> T cells. Myr+ and Myr− CD4+ T-cell lines were generated as described in Materials and Methods. (A and B) Cell lysates from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were prepared by lysing of cells in radioimmunoprecipitation assay (RIPA) lysis buffer. The cell lysates were separated by SDS–12% PAGE and transferred to two nitrocellulose filters and then immunoblotted with an HIV-1-positive human serum (A) or with a monoclonal anti-RTp66/p51 antibody (B). (C) The supernatants from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were harvested after 48 h of incubation with fresh complete medium. The virion-associated proteins from harvested supernatants were concentrated by ultracentrifugation and analyzed by SDS-PAGE and immunoblotting with an HIV-1-positive human serum. (D) Cell lysates from uninfected H9 (lane 1), Myr−/H9 (lane 2), and Myr+/H9 (lane 3) cell lines were prepared by lysing the cells in RIPA lysis buffer. The cell lysates were separate by SDS–12% PAGE and the immunoblotted with an HIV-1-positive human serum.
    Cd4 T Lymphoid Cell Line Supt, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4 t lymphoid cell line supt/product/ATCC
    Average 97 stars, based on 646 article reviews
    cd4 t lymphoid cell line supt - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "A Bipartite Membrane-Binding Signal in the Human Immunodeficiency Virus Type 1 Matrix Protein Is Required for the Proteolytic Processing of Gag Precursors in a Cell Type-Dependent Manner"

    Article Title: A Bipartite Membrane-Binding Signal in the Human Immunodeficiency Virus Type 1 Matrix Protein Is Required for the Proteolytic Processing of Gag Precursors in a Cell Type-Dependent Manner

    Journal:

    doi:

    Defect in proteolytic processing of HIV-1 Gag precursors in Myr− CD4+ T cells. Myr+ and Myr− CD4+ T-cell lines were generated as described in Materials and Methods. (A and B) Cell lysates from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were prepared by lysing of cells in radioimmunoprecipitation assay (RIPA) lysis buffer. The cell lysates were separated by SDS–12% PAGE and transferred to two nitrocellulose filters and then immunoblotted with an HIV-1-positive human serum (A) or with a monoclonal anti-RTp66/p51 antibody (B). (C) The supernatants from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were harvested after 48 h of incubation with fresh complete medium. The virion-associated proteins from harvested supernatants were concentrated by ultracentrifugation and analyzed by SDS-PAGE and immunoblotting with an HIV-1-positive human serum. (D) Cell lysates from uninfected H9 (lane 1), Myr−/H9 (lane 2), and Myr+/H9 (lane 3) cell lines were prepared by lysing the cells in RIPA lysis buffer. The cell lysates were separate by SDS–12% PAGE and the immunoblotted with an HIV-1-positive human serum.
    Figure Legend Snippet: Defect in proteolytic processing of HIV-1 Gag precursors in Myr− CD4+ T cells. Myr+ and Myr− CD4+ T-cell lines were generated as described in Materials and Methods. (A and B) Cell lysates from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were prepared by lysing of cells in radioimmunoprecipitation assay (RIPA) lysis buffer. The cell lysates were separated by SDS–12% PAGE and transferred to two nitrocellulose filters and then immunoblotted with an HIV-1-positive human serum (A) or with a monoclonal anti-RTp66/p51 antibody (B). (C) The supernatants from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were harvested after 48 h of incubation with fresh complete medium. The virion-associated proteins from harvested supernatants were concentrated by ultracentrifugation and analyzed by SDS-PAGE and immunoblotting with an HIV-1-positive human serum. (D) Cell lysates from uninfected H9 (lane 1), Myr−/H9 (lane 2), and Myr+/H9 (lane 3) cell lines were prepared by lysing the cells in RIPA lysis buffer. The cell lysates were separate by SDS–12% PAGE and the immunoblotted with an HIV-1-positive human serum.

    Techniques Used: Generated, Radio Immunoprecipitation, Lysis, Incubation, SDS Page, Western Blot



    Similar Products

    cd4 t lymphoid cell line supt  (ATCC)
    97
    ATCC cd4 t lymphoid cell line supt
    Defect in proteolytic processing of HIV-1 Gag precursors in Myr− <t>CD4+</t> T cells. Myr+ and Myr− CD4+ T-cell lines were generated as described in Materials and Methods. (A and B) Cell lysates from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were prepared by lysing of cells in radioimmunoprecipitation assay (RIPA) lysis buffer. The cell lysates were separated by SDS–12% PAGE and transferred to two nitrocellulose filters and then immunoblotted with an HIV-1-positive human serum (A) or with a monoclonal anti-RTp66/p51 antibody (B). (C) The supernatants from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were harvested after 48 h of incubation with fresh complete medium. The virion-associated proteins from harvested supernatants were concentrated by ultracentrifugation and analyzed by SDS-PAGE and immunoblotting with an HIV-1-positive human serum. (D) Cell lysates from uninfected H9 (lane 1), Myr−/H9 (lane 2), and Myr+/H9 (lane 3) cell lines were prepared by lysing the cells in RIPA lysis buffer. The cell lysates were separate by SDS–12% PAGE and the immunoblotted with an HIV-1-positive human serum.
    Cd4 T Lymphoid Cell Line Supt, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4 t lymphoid cell line supt/product/ATCC
    Average 97 stars, based on 1 article reviews
    cd4 t lymphoid cell line supt - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Defect in proteolytic processing of HIV-1 Gag precursors in Myr− CD4+ T cells. Myr+ and Myr− CD4+ T-cell lines were generated as described in Materials and Methods. (A and B) Cell lysates from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were prepared by lysing of cells in radioimmunoprecipitation assay (RIPA) lysis buffer. The cell lysates were separated by SDS–12% PAGE and transferred to two nitrocellulose filters and then immunoblotted with an HIV-1-positive human serum (A) or with a monoclonal anti-RTp66/p51 antibody (B). (C) The supernatants from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were harvested after 48 h of incubation with fresh complete medium. The virion-associated proteins from harvested supernatants were concentrated by ultracentrifugation and analyzed by SDS-PAGE and immunoblotting with an HIV-1-positive human serum. (D) Cell lysates from uninfected H9 (lane 1), Myr−/H9 (lane 2), and Myr+/H9 (lane 3) cell lines were prepared by lysing the cells in RIPA lysis buffer. The cell lysates were separate by SDS–12% PAGE and the immunoblotted with an HIV-1-positive human serum.

    Journal:

    Article Title: A Bipartite Membrane-Binding Signal in the Human Immunodeficiency Virus Type 1 Matrix Protein Is Required for the Proteolytic Processing of Gag Precursors in a Cell Type-Dependent Manner

    doi:

    Figure Lengend Snippet: Defect in proteolytic processing of HIV-1 Gag precursors in Myr− CD4+ T cells. Myr+ and Myr− CD4+ T-cell lines were generated as described in Materials and Methods. (A and B) Cell lysates from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were prepared by lysing of cells in radioimmunoprecipitation assay (RIPA) lysis buffer. The cell lysates were separated by SDS–12% PAGE and transferred to two nitrocellulose filters and then immunoblotted with an HIV-1-positive human serum (A) or with a monoclonal anti-RTp66/p51 antibody (B). (C) The supernatants from uninfected SupT-1 (lane 1), Myr−/SupT-1 (lane 2), and Myr+/SupT-1 (lane 3) cell lines were harvested after 48 h of incubation with fresh complete medium. The virion-associated proteins from harvested supernatants were concentrated by ultracentrifugation and analyzed by SDS-PAGE and immunoblotting with an HIV-1-positive human serum. (D) Cell lysates from uninfected H9 (lane 1), Myr−/H9 (lane 2), and Myr+/H9 (lane 3) cell lines were prepared by lysing the cells in RIPA lysis buffer. The cell lysates were separate by SDS–12% PAGE and the immunoblotted with an HIV-1-positive human serum.

    Article Snippet: The CD4 + T-lymphoid cell line SupT-1 was also obtained from the American Type Culture Collection and was maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum and antibiotics.

    Techniques: Generated, Radio Immunoprecipitation, Lysis, Incubation, SDS Page, Western Blot